Open AccessApplication of Proteomics in Biomarker Discovery: a Primer for the Clinician
Author(s) -
Vojtěch Tambor,
Alena Fučíková,
Juraj Lenčo,
Marian Kacerovský,
V Řeháček,
Jiřı́ Stulı́k,
R Pudil
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931758
Subject(s) - biomarker discovery , proteomics , biomarker , biomedicine , computational biology , data science , drug discovery , computer science , bioinformatics , medicine , biology , biochemistry , gene
Ever since proteomics was proven to be capable of characterizinga large number of differences in both protein quality andquantity, it has been applied in various areas of biomedicine,ranging from the deciphering molecular pathogenesis of diseasesto the characterization of novel drug targets and the discovery ofpotential diagnostic biomarkers. Indeed, the biomarker discoveryin human plasma is clearly one of the areas with enormouspotential. However, without proper planning and implementationof specific techniques, the efforts and expectations may veryeasily be hampered. Numerous earlier projects aimed at clinicalproteomics, characterized by exaggerated enthusiasm, oftenunderestimated some principal obstacles of plasma biomarkerdiscovery. Consequently, ambiguous and insignificant resultssoon led to a more critical view in this field. In this article, wecritically review the current state of proteomic approaches forbiomarker discovery and validation, in order to provide basicinformation and guidelines for both clinicians and researchers.These need to be closely considered prior to initiation of a projectaimed at plasma biomarker discovery. We also present a shortoverview of recent applications of clinical proteomics inbiomarker discovery.