Open AccessParvalbumin and TRPV1 receptor expression in dorsal root ganglion neurons after acute peripheral inflammation
Author(s) -
Gisela Zachařová,
J. Paleček
Publication year - 2009
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931738
Subject(s) - trpv1 , dorsal root ganglion , parvalbumin , receptor , neurotransmission , neuroscience , spinal cord , chemistry , microbiology and biotechnology , transient receptor potential channel , medicine , endocrinology , biology
Expression of parvalbumin (PV) and transient receptor potentialvanilloid (TRPV1) receptors in the lumbar dorsal root ganglionneurons (DRG) was evaluated in control animals and in rats afteracute carageenan-induced knee joint inflammation. PV is acalcium binding protein that acts as a calcium buffer, affectsintracellular calcium homeostasis and may thus influence signaltransduction and synaptic transmission. TRPV1 receptors areviewed as molecular integrators of nociceptive stimuli andmodulate spinal cord synaptic transmission beside their functionin the peripheral nerve endings. In naive rats, 13 % of the L4DRG neurons had PV immunopositivity (PV+) and 36 %expressed TRPV1 receptors (TRPV1+). The soma of the PV+neurons was of medium to large size, while the TRPV1 receptorswere expressed in small diameter neurons. The co-localization ofthe PV and TRPV1 immunoreactivity was minimal (0.2 %). Therewas no significant change in the PV+ (11 %), TRPV1+ (42 %)and PV+TRPV1+ (0.25 %) expression, or shift in the neuronalsize distribution 28 h after the unilateral peripheral inflammation,both when compared to controls and when ipsilateral tocontralateral sides were evaluated. Thus under the givenexperimental conditions, no change in somatic TRPV1 receptorsand PV expression in L4 DRG neurons was found.