Open AccessThe Influence of Obesity and Different Fat Depots on Adipose Tissue Gene Expression and Protein Levels of Cell Adhesion Molecules
Author(s) -
Lenka Bošanská,
David Michalský,
Z Lacinová,
I Dostálová,
Markéta Bártlová,
D Haluzíková,
Martin Matoulek,
M Kasalický,
Martin Haluzı́k
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931705
Subject(s) - cell adhesion molecule , adipose tissue , medicine , endocrinology , vcam 1 , e selectin , intercellular adhesion molecule 1 , cell adhesion , icam 1 , endothelial activation , adhesion , chemistry , inflammation , immunology , organic chemistry
Increased circulating adhesion molecules in patients with obesityplay an important role in the development of endothelialdysfunction/atherosclerosis. The aim of this study was to assessthe contribution of various fat depots to the production ofadhesion molecules in obesity. 12 women with 1st and 2nddegree of obesity, 13 women with 3rd degree of obesity and14 lean age-matched women were included into study.Circulating levels of vascular cell adhesion molecule-1 (VCAM-1),intercellular adhesion molecule-1 (ICAM-1), and E-selectin weremeasured by Luminex kits. mRNA expression of ICAM-1, VCAM-1,E-selectin, monocyte chemoattractant protein-1 (MCP-1), andCD68 in subcutaneous (SAT) and visceral adipose tissue (VAT)was measured by RT-PCR; ICAM-1 and VCAM-1 protein levels byLuminex kits, normalized to protein content. Obesity increasedICAM-1 and VCAM-1 mRNA expression and protein levels andCD68 mRNA expression in VAT. Expression of E-selectin andMCP-1 did not significantly differ between groups. Expression ofICAM-1 and VCAM-1 positively correlated with expression ofCD68 in both adipose depots. In VAT, ICAM-1 and VCAM-1expression and protein levels positively correlated with BMI.Obesity was associated with increased adhesion molecules mRNAexpression and protein levels in VAT, but not in SAT. Increasedadhesion molecules production in visceral fat may provide a noveldirect link between visceral adiposity and increased risk ofcardiovascular complications.