Open AccessDifferential regulation of galectin expression/reactivity during wound healing in porcine skin and in cultures of epidermal cells with functional impact on migration
Author(s) -
Jiří Klíma,
Lukáš Lacina,
Barbora Dvořánková,
Doris Herrmann,
J.W. Carnwath,
Hartmut H. Niemann,
Herbert Kaltner,
Sabine André,
Jan Motlı́k,
HansJoachim Gabius,
Karel Smetana
Publication year - 2009
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931624
Subject(s) - galectin , wound healing , microbiology and biotechnology , biology , epidermis (zoology) , blot , cell adhesion , cell migration , lectin , dermis , galectin 1 , downregulation and upregulation , cell , immunology , biochemistry , anatomy , gene
The glycophenotyping of mammalian cells with plant lectins mapsaspects of the glycomic profile and disease-associated alterations.A salient step toward delineating their functional dimension is thedetection of endogenous lectins. They can translate sugar-encodedchanges into cellular responses. Among them, the members of thelectin family of galectins are emerging regulators of cell adhesion,migration and proliferation. Focusing on galectins-1, -3 and -7, weaddressed the issue whether their expression is regulated duringwound healing in porcine skin as model. A conspicuousupregulation is detected for galectin-1 in the dermis and aneoexpression in the epidermis, where an increased level ofgalectin-7 was also found. Applying biotinylated tissue lectins asprobes, the signal intensities for accessible binding sites decreased,intimating an interaction of the cell lectin with reactive sites. Incontrast, galectin-3 parameters remained rather constant. Of note,epidermal cells in culture also showed an increase inexpression/presence of galectin-1, measured on the levels ofmRNA and protein, in this case by Western blotting andquantitative immunocytochemistry. Used as matrix, galectin-1conferred resistance to trypsin treatment to attached humankeratinocytes and reduced migration into scratch-wound areas invitro. This report thus presents new information on endogenouslectins in wound healing and differential regulation among thethree tested cases.