Open AccessEffects of Anserine on the Renal Sympathetic Nerve Activity and Blood Pressure in Urethane-Anesthetized Rats
Author(s) -
Mamoru Tanida,
Jiao Shen,
Daiki Kubomura,
Katsuya omura
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931623
Subject(s) - anserine , histaminergic , carnosine , medicine , endocrinology , blood pressure , chemistry , antagonist , anesthesia , receptor , histidine , amino acid , biochemistry
Previous studies have demonstrated that central injection ofL-carnosine (β-alynyl-L-histidine), dipeptide synthesized inmammalian muscles, affects renal sympathetic nerve activity(RSNA) and blood pressure (BP) in anesthetized rats. In thepresent study, using urethane-anesthetized rats, we examinedthe dose-dependent effects of intravenous (IV) injection ofvarious doses of anserine, dipeptide of similar structure toL-carnosine, on RSNA, BP and heart rate (HR). We found thatinjection of a low dose of anserine (1 μg) significantly suppressedRSNA, BP and HR. Conversely, a high dose (1000 μg) of anserinesignificantly elevated RSNA, BP and HR. Pretreatment with lateralcerebral ventricular (LCV) injection of thioperamide, ahistaminergic H3-receptor antagonist, eliminated the effects of alow dose of anserine on RSNA, BP and HR. LCV injection ofdiphenhydramine, a histaminergic H1-receptor antagonist,abolished the effects of a high dose of anserine on RSNA, BP andHR. These findings suggest that anserine affects RSNA, BP andHR in a dose-dependent manner, and that the histaminergicnerve may be involved in the dose-different effects of anserine inrats.