Open AccessThe effects of pertussis toxin-treatment on integrated vasoactive response of vascular system in spontaneously hypertensive rats
Author(s) -
Soňa Čačányiová,
František Kristek,
Jaroslav Kuneš,
J Zicha
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931576
Subject(s) - pressor response , pertussis toxin , bradykinin , endocrinology , medicine , blood pressure , vasoactive , acetylcholine , spontaneously hypertensive rat , heart rate , g protein , receptor
We investigated the effect of pertussis toxin (PTX) onhypotensive response induced by acetylcholine (ACh) andbradykinin (BK) and on noradrenaline (NA)-induced pressorresponse in spontaneously hypertensive rats (SHR). Fifteenweek-old Wistar rats and age-matched SHR were used. Half of SHR received PTX (10 µg/kg/i.v.) and the experiments were performed 48 h later. After the anesthesia the right carotid arterywas cannulated in order to record blood pressure (BP). Thehypotensive response to ACh was enhanced in SHR compared toWistar rats. After pretreatment of SHR with PTX the hypotensiveresponse to ACh was reduced compared to untreated SHR and itwas also diminished in comparison to Wistar rats. Similarly, thehypotensive response to BK was also decreased after PTXpretreatment. The pressor response to NA was increased in SHRcompared to Wistar rats. NA-induced pressor response wasconsiderably decreased after PTX pretreatment compared tountreated SHR. In conclusion, the enhancement of hypotensiveand pressor responses in SHR was abolished after PTXpretreatment. Our results suggested that the activation of PTXsensitive inhibitory Gi proteins is involved in the regulation ofintegrated vasoactive responses in SHR and PTX pretreatmentcould be effectively used for modification of BP regulation in thistype of experimental hypertension.