Open AccessEndogenous Protective Mechanisms in Remodeling of Rat Heart Mitochondrial Membranes in the Acute Phase of Streptozotocin-Induced Diabetes
Author(s) -
M. Ferko,
D Habodászová,
Iveta Waczulı́ková,
Jana Mujkosová,
J Kucharská,
L Šikurová,
B Ziegelhöffer,
J Styk,
A Ziegelhöffer
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931554
Subject(s) - streptozotocin , mitochondrion , medicine , endocrinology , membrane fluidity , diabetes mellitus , chemistry , endogeny , respiration , inner mitochondrial membrane , reactive oxygen species , biology , membrane , biochemistry , anatomy
The aim of present study was to investigate functional and physical alterations in membranes of heart mitochondria that are associated with remodeling of these organelles in acute phase of streptozotocin-induced diabetes and to elucidate the role of these changes in adaptation of the heart to acute streptozotocin-induced diabetes (evaluated 8 days after single dose streptozotocin application to male Wistar rats). Action of free radicals on the respiratory chain of diabetic-heart mitochondria was manifested by 17 % increase (p 0.05). On the contrary, membrane fluidity and the total mitochondrial Mg2+-ATPase activity increased (both p<0.05). In diabetic heart mitochondria, linear regression analysis revealed a reciprocal relationship between the increase in membrane fluidity and decrease in trans-membrane potential (p<0.05, r = 0.67). Changes in membrane fluidity, transmembrane potential, Mg2+-ATPase activity and the almost preserved ADP/O ratio appear as the manifestation of endogenous protective mechanisms participating in the functional remodeling of mitochondria which contributes to adaptation of the heart to diabetes.