Open AccessAnorexigenic effect of cholecystokinin is lost but that of CART (Cocaine and Amphetamine Regulated Transcript) peptide is preserved in monosodium glutamate obese mice
Author(s) -
Blanka Železná,
Jana Maixnerová,
Resha Matyšková,
Renata Haugvicová,
Darja Blokešová,
Lenka Maletı́nská
Publication year - 2009
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931511
Subject(s) - orexigenic , monosodium glutamate , cart , endocrinology , medicine , cholecystokinin , neuropeptide y receptor , cocaine and amphetamine regulated transcript , leptin , arc (geometry) , neuropeptide , appetite , chemistry , obesity , receptor , mechanical engineering , geometry , mathematics , engineering
Monosodium glutamate (MSG) treatment of neonatal mice resultsin a selective damage to the arcuate nucleus (ARC) anddevelopment of obesity with increased adiposity at sustainedbody weight in the adulthood. Feeding pattern of the MSG obesemice is unusual. Our previous results showed that after 24-hfasting, MSG mice consumed negligible amount of food in severalhours and therefore, it was impossible to register the effect ofpeptides attenuating food intake such as cholecystokinin (CCK) orcocaine- and amphetamine-regulated transcript (CART) peptide.To overcome this problem, two findings were used: firstly,orexigenic effect of neuropeptide Y (NPY) was attenuated bothby CCK or CART peptide in lean fed mice and secondly,orexigenic effect of NPY was preserved in fed rats with MSGobesity. In this study, short-term food intake in fed lean and MSGobese C57BL/6 male mice was measured after simultaneouscentral administration of orexigenic NPY with either CART peptideor peripherally administered CCK. Anorexigenic action ofexogenous CART peptide was preserved in MSG obese mice. Onthe other hand, satiety effect of exogenous CCK was completelylost in MSG obese mice. In conclusion, effective leptin signalingin ARC is necessary for satiety effect of CCK.