Open AccessAge-dependent expression of 5alpha-reductase and androgen receptors mRNA by the canine prostate
Author(s) -
Falah Shidaifat
Publication year - 2009
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931358
Subject(s) - dihydrotestosterone , prostate , androgen receptor , prostate cancer , androgen , testosterone (patch) , endocrinology , medicine , hyperplasia , gene expression , biology , messenger rna , gene , cancer , hormone , genetics
The growth of the prostate gland is androgen-dependent.Testosterone is converted to the most potent dihydrotestosterone(DHT) by 5α-reductase within the prostate. Androgen interactswith androgen receptors (AR) to regulate normal growth of theprostate and has also been implicated in both the progression ofbenign prostate hyperplasia and prostate cancer. This study wasconducted to compare the mRNA expression of AR and 5αreductase by the prostate gland from three age categories:immature, young-mature and old dogs. Quantitative geneexpression was assessed by the real-time PCR and the resultswere expressed as a relative mRNA expression of the targetgene. This study revealed that there was no significant differencein the mRNA expression of the AR gene by the prostate gland ofimmature, young and old dogs. In contrast, there is a highlysignificant (P<0.001) down-regulation in 5α-reductase gene bythe prostate of young and old dogs as compared with immaturedogs. However, there is no significant difference in mRNAexpression of the 5α-reductase gene by the prostate gland fromyoung and old dogs. This differential expression of AR and 5αreductase genes, which are involved in the regulation ofandrogen effect on prostate gland, might reflect an agedependent growth requirement of the gland for androgens.