Open AccessIn situ assessment of the liver microcirculation in mechanically ventilated rats using sidestream dark-field imaging
Author(s) -
V. Černý,
Z. Turek,
R Pařízková
Publication year - 2009
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931353
Subject(s) - microcirculation , biomedical engineering , medicine , in situ , pathology , radiology , chemistry , organic chemistry
Assessment of hepatic microcirculation by on-line visualizationhas been impossible for a long time. Sidestream dark-field (SDF)imaging is a relatively new method allowing direct visualization ofboth mucosal microcirculation and surface layers microcirculationof solid organs using hand-held probe for direct contact withtarget tissue. The aim of this study was to evaluate the feasibilityof studying the rat hepatic microcirculation in situ by SDFimaging. The liver lobes were left in situ, and images wereobtained using SDF imaging on the surface of the liver via uppermidline laparotomy. Images were captured intermittently during10-sec apnoea and recorded. The microvascular parameters werecompared with previous validation studies. Clear high contrastSDF images were successfully obtained. Quantitative analysisrevealed a mean FSD (functional sinusoidal density) of 402±15cm/cm2, a sinusoidal diameter of 10.2±0.5 μm and postsinusoidalvenular diameter of 33.9±13 μm. SDF imaging is a suitablenoninvasive method for accurate quantification of the basicmicrocirculatory parameters of the liver in situ without a need toexteriorize the liver lobes. This method seems to be applicable inanimal studies with possibility to use SDF imaging alsointraoperatively, providing unique opportunity to study livermicrocirculation during various experimental and clinical settings.