Open AccessInfluence of gemfibrozil on sulfate transport in human erythrocytes during the oxygenation-deoxygenation cycle
Author(s) -
Ester Tellone,
Silvana Ficarra,
Roberto Scatena,
Bruno Giardina,
Arnošt Kotyk,
Annamaria Russo,
Deborah Colucci,
Ersilia Bellocco,
Giuseppina Laganà,
Antonio Galtieri
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931251
Subject(s) - chemistry , gemfibrozil , deoxygenation , band 3 , biophysics , biochemistry , erythrocyte membrane , membrane , cholesterol , biology , catalysis
The effects of gemfibrozil (GFZ), an antihyperlipidemic agent, onthe anionic transport of the human red blood cells (RBC) duringthe oxygenation-deoxygenation cycle were examined. Gemfibrozilclearly plays a role in the modulation of the anionic flux inerythrocytes; in fact it causes a strong increment of anionstransport when the RBCs are in the high-oxygenation state(HOS). Such an effect is remarkably reduced in the lowoxygenation state (LOS). With the aim of identifying the dynamics of fibrate action, this effect has been investigated alsoin human ghost and chicken erythrocytes. These latter, in fact,are known to possess a B3 (anion transporter or Band 3)modified at the cytoplasmic domain (cdb3) which plays asignificant role in the metabolic modulation of red blood cells.The results were analyzed taking into account the well-knowninteractions between fibrates and both conformational states ofhemoglobin i.e. the T state (deoxy-conformation) and the R state(oxy-conformation). The effect of gemfibrozil on anionic influxappears to be due to a wide interaction involving a “multimeric”Hb-GFZ-cdb3 macromolecular complex.