Open AccessLipoprotein lipase activity determined in vivo is lower in carriers of apolipoprotein A-V gene variants 19W and -1131C
Author(s) -
J. Kovář,
Věra Adámková
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931245
Subject(s) - nefa , endocrinology , medicine , lipoprotein lipase , apolipoprotein b , triglyceride , hypertriglyceridemia , population , heterozygote advantage , chemistry , biology , insulin , cholesterol , gene , genotype , adipose tissue , biochemistry , environmental health
The apolipoprotein A-V (apo A-V) plays an important role inregulation of triglyceride (TG) concentration in serum. To betterunderstand how apo A-V affects triglyceridemia andglucoregulation, the lipoprotein lipase (LPL) activity wasdetermined using intravenous fat tolerance test (IVFTT) and oralglucose tolerance test (oGTT) was performed in carriers ofapolipoprotein A-V gene (APOAV) variants known to beassociated with increased triglyceridemia. Twelve carriers of 19Wvariant, 16 carriers of -1131C variant, 1 combined heterozygoteand 16 control subjects homozygous for wild type variants (19S/-1131T) were selected from a population sample and matchedwith respect to body mass index and age. The APOAV variantscarriers had increased TG, very low density lipoprotein-TG, andapo B concentrations (p < 0.05). The LPL activity evaluated as k2rate constant for clearance of Intralipid® was 14 % lower inAPOAV variants carriers. The depression of nonesterified fattyacids (NEFA) concentration after glucose load was delayed inAPOAV variants carriers in spite of the same insulinemia andglycemia. Our results suggest that variants of APOAV combinedwith increased triglyceridemia are associated with lower LPLactivity in vivo and with disturbances of regulation of NEFAconcentration after glucose load.