Open AccessRole of ghrelin in the regulation of gastric acid secretion involving nitrergic mechanisms in rats
Author(s) -
HM Bilgin,
Cemıl Tümer,
Hüda Diken,
Mustafa Kelle,
Abdurrahman Şermet
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931234
Subject(s) - ghrelin , medicine , endocrinology , gastric acid , secretion , secretagogue , nitrite , nitric oxide , chemistry , mucus , growth hormone secretagogue receptor , hormone , biology , ecology , organic chemistry , nitrate
Ghrelin, an endogenous ligand for growth hormone secretagoguereceptor (GHS-R), has been identified in the rat and humangastrointestinal tract. Ghrelin has been proposed to play a role ingastric acid secretion. Nitric oxide (NO) was shown as a mediatorin the mechanism of ghrelin action on gastric acid secretoryfunction. However, there is a little knowledge about this topic.We have investigated the role of ghrelin in gastric acid secretionand the role of NO as a mediator. Wistar albino rats were used inthis study. The pyloric sphincter was ligated through a smallmidline incision. By the time, saline (0.5 ml, iv) was injected tothe control group, ghrelin (20 μg/kg, iv) was injected to the firstexperimental group, ghrelin (20 μg/kg, iv) + L-NAME (70 mg/kg,sc) was injected to the second group and L-NAME (70 mg/kg, sc)was administered to the third group. The rats were killed 3 hafter pylorus ligation; gastric acid secretion, mucus content andplasma nitrite levels were measured. Exogenous ghrelinadministration increased gastric acid output, mucus content andtotal plasma nitrite levels, while these effects of ghrelin wereinhibited by applying L-NAME. We can conclude that ghrelinparticipates in the regulation of gastric acid secretion through NOas a mediator.