Open AccessImmunological response in the mouse melanoma model after local hyperthermia
Author(s) -
Jiří Kubeš,
J Svoboda,
Jozef Rosina,
M Starec,
Anna Fišerová
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.93121
Subject(s) - splenocyte , cytotoxic t cell , spleen , cd8 , melanoma , flow cytometry , tumor microenvironment , cancer research , immunology , biology , chemistry , in vitro , microbiology and biotechnology , immune system , biochemistry
Our study was aimed to characterize the phenotype andfunctional endpoints of local microwave hyperthermia (LHT,42 °C) on tumor infiltrating and spleen leukocytes. Theeffectiveness of LHT applied into the tumor of B16F10melanoma-bearing C57/BL6 mice was compared withanesthetized and non-treated animals. Subpopulations ofleukocytes were analyzed using the flow cytometry, and thecytotoxic activity of splenocytes against syngeneic B16F10melanoma and NK-sensitive YAC-1 tumor cell lines was evaluatedin 51Cr-release assay. Similarly, the in vitro modification of theheat treatment was performed using healthy and melanomabearing splenocytes. We found a 40 % increase of activatedmonocytes (CD11b+CD69+) infiltration into the tumormicroenvironment. In the spleen of experimental animals, thenumbers of cytotoxic T lymphocytes (CTLs-CD3+CD8+) and NKcell (CD49b+NK1.1+) raised by 22 % and 14 %, respectively,while the NK1.1+ monocytes decreases by 37 %. This wasaccompanied by an enhancement of cytotoxic effector functionagainst B16F10 and YAC-1 targets in both in vivo and in vitroconditions. These results demonstrate that LHT induces betterkilling of syngeneic melanoma targets. Furthermore, LHT evokesthe homing of activated monocytes into the tumormicroenvironment and increases the counts of NK cells and CTLin the spleen.