Open AccessBand 3 protein function in teleost fish erythrocytes: effect of oxygenation-deoxygenation
Author(s) -
Annamaria Russo,
Ester Tellone,
Silvana Ficarra,
Bruno Giardina,
Ersilia Bellocco,
Giuseppina Laganà,
Ugo Leuzzi,
Arnošt Kotyk,
Antonio Galtieri
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931178
Subject(s) - hemoglobin , oxygenation , band 3 , bicarbonate , chemistry , vertebrate , biochemistry , deoxygenation , biophysics , tuna , oxygen transport , red blood cell , fish physiology , biology , oxygen , fish <actinopterygii> , membrane , membrane protein , endocrinology , fishery , ecology , organic chemistry , gene , catalysis
During vertebrate evolution, structural changes in red blood cells(RBC) and hemoglobin (Hb), have probably resulted in theimportance of blood carbon dioxide transport. Thechloride/bicarbonate exchange across the RBC membrane, whichis an integral part of the blood CO2 transport process invertebrates, has been examined on two different species ofteleost fish, Euthynnus alletteratus and Thunnus thynnus, atseveral oxygenation states of erythrocyte HOS (high-oxygenationstate, about 90 % of saturation) and LOS (low-oxygenation state,about 15 % of saturation). The results were compared with thoseobserved in human RBC under the same experimental conditionsand with the chicken (Gallus gallus) erythrocytes, which haveparticular modifications at the N-terminus of the band 3 protein(B3). In fish the kinetic measurements have shown a differentanion transport in several oxygenation states of erythrocytes,indicating that also at lower levels of vertebrate evolution thereexists a modulation of the anionic flow affected by oxygen. Thefunctional correlation of anion transport to changes of parts ofthe hemoglobin sequence responsible for alterations in theinteractions with the cytoplasmic domain of band 3 protein(cdb3) allowed us to suggest a hypothesis about fish physiology.The highest values of kinetic measurements observed in fishhave been attributed to the metabolic need of the RBC inresponse to the removal of CO2 that in teleosts is also ofendogenous origin.