Open AccessLeptin affects proliferation-, apoptosis- and protein kinase A-related peptides in human ovarian granulosa cells
Author(s) -
Sirotkin Av,
M Mlyncek,
Makarevich Av,
Iveta Florkovičová,
L. Hetényi
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931153
Subject(s) - leptin , apoptosis , intracellular , proliferating cell nuclear antigen , in vitro , immunocytochemistry , cell growth , protein kinase a , endocrinology , kinase , medicine , cyclin , chemistry , biology , microbiology and biotechnology , cell cycle , biochemistry , obesity
The aim of our in vitro studies was to understand the role ofleptin in controlling proliferation, apoptosis, and protein kinase A(PKA) in human ovarian cells. We analyzed the in vitro effects ofleptin (0, 1, 10 or 100 ng/ml) on the accumulation ofproliferation-related peptides (PCNA, cyclin B1), apoptosisassociated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells usingimmunocytochemistry and Western immunoblotting. It wasobserved that leptin stimulated in a dose-dependent manner theaccumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (atdoses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA(at doses 1-100 ng/ml) in cultured human ovarian cells. Theseobservations suggest the ability of leptin to control directlyhuman ovarian cell functions: proliferation, apoptosis, andintracellular messenger PKA.