Open AccessSome endocrine traits of transgenic rabbits. II. Changes in hormone secretion and response of isolated ovarian tissue to FSH and ghrelin
Author(s) -
Alexander V. Sirotkin,
Peter Chrenek,
K. Darłak,
Francisco Valenzuela,
Z. Kuklova
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931150
Subject(s) - ghrelin , endocrinology , medicine , transgenesis , hormone , biology , transgene , secretion , endocrine system , genetically modified mouse , follicle stimulating hormone , reproductive technology , luteinizing hormone , gene , embryogenesis , biochemistry
In the present in vitro experiments we examined FSH- andghrelin-induced changes in ovarian hormone secretion bytransgenic rabbits. Fragments of ovaries isolated from adulttransgenic (carrying mammary gland-specific mWAP-hFVIIIgene) and non-transgenic rabbits from the same litter werecultured with and without FSH or ghrelin (both at 0, 1, 10 or 100ng/ml medium). The secretion of progesterone (P4), estradiol (E2)and insulin-like growth factor I (IGF-I) was assessed by RIA. Itwas observed that ovaries isolated from transgenic rabbitssecreted much less P4, E2 and IGF-I than the ovaries of nontransgenic animals. In control animals FSH reduced E2 (at doses1-100 ng/ml medium) and IGF-I (at 1-100 ng/ml), but not P4secretion, whereas ghrelin promoted P4 (at 1 ng/ml) and IGF-I(at 100 ng/ml), but not E2 output. In transgenic animals, theeffects were reversed: FSH had a stimulatory effect on E2 (at 100ng/ml) and ghrelin had an inhibitory effect on P4 (at 10 ng/ml).No differences in the pattern of influence of FSH on P4 and IGF-Iand of ghrelin on E2 and IGF-I were found between control andtransgenic animals. The present observations suggest that1) both FSH and ghrelin are involved in rabbit ovarian hormonesecretion, 2) transgenesis in rabbits is associated with areduction in ovarian secretory activity, and 3) transgenesis canaffect the response of ovarian cells to hormonal regulators.