Open AccessOntogeny of reactive nitrogen species production by blood phagocytes in pigs
Author(s) -
Petra Zelníčková,
Martin Faldyna,
Jaroslav Ondracek,
H Kovářů,
F Kovářů
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931141
Subject(s) - ontogeny , gestation , nitrotyrosine , weaning , nitric oxide , reactive nitrogen species , biology , fetus , medicine , andrology , endocrinology , immunology , nitric oxide synthase , pregnancy , genetics
The aim of this work was to evaluate ontogeny of reactivenitrogen species (RNS) production by peripheral bloodphagocytes in pig. Pig fetuses (55 and 92 days of gestation) andpostnatal piglets (1, 3, 8, 17, 31 and 41 days after birth) wereused. RNS production was measured by fluorescent probesdiaminofluorescein-diacetate (DAF-FMDA) and dichlorofluorescein-diacetate (H2DCFDA). Levels of nitration of cell proteins were established by immunofluorescent detection ofnitrotyrosine. Levels of plasma nitrites/nitrates were detectedspectrophotometrically by Griess reaction. Nitric oxide productionmeasured by DAF-FMDA in neutrophils decreased duringpostnatal life. Spontaneous RNS measured by H2DCFDAdecreased from 55th day of gestation to the 41st day of life.Phorbol-12-myristate-13-acetate activated production decreasedduring postnatal life. Production of NO measured by DAF-FMDAin macrophages decreased from the 1st to 41st day after birth.RNS production measured by H2DCFDA in monocytes did notshow any significant changes during ontogeny. The level ofnitrotyrosine significantly decreased from the 3rd to 17th day.Levels of plasma nitrites/nitrates gradually decreased from the55th day of gestation to the 41st day after birth. A temporaryincrease in all parameters occurred after weaning, but withoutany significance. In conclusion, RNS production has a decreasingtrend during ontogeny and is transiently upregulated afterweaning.