Open AccessDifferent functional responsibility of the small intestine to high-fat/high-energy diet determined the expression of obesity-prone and obesity-resistant phenotypes in rats
Author(s) -
Zuzana Šefčíková,
Tomáš Hájek,
L Lenhardt,
Ľ Raček,
Š Mózeš
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931117
Subject(s) - medicine , endocrinology , obesity , energy homeostasis , small intestine , alkaline phosphatase , weight gain , biology , chemistry , enzyme , body weight , biochemistry
The objective of the present experiment was to assess theinvolvement of small intestine in expression of susceptibility orresistance to the high-fat/high-energy diet. The investigation wascarried out in adult male Sprague-Dawley rats fed either standardlaboratory diet (3.2 kcal/g, 9.5 % fat) or high-fat (HF) diet (4.04kcal/g, 30 % fat) for 4 weeks as well as in HF rats that wereretrospectively designated on the bases of their higher or lowerweight gain as sensitive (DIO) or resistant (DR) to obesity. Ourresults revealed in HF group significant increase in energy intake,food efficiency, weight gain and Lee´s index of obesity.Moreover, in comparison with controls, a significantly increasedduodenal and jejunal alkaline phosphatase (AP) andα-glucosidase activity as well as hypertrophy of jejunal mucosa(increased protein/DNA ratio) were observed in HF fed rats. Incontrast, intestinal function was inversely related to energyintake or to the development of adiposity in DIO vs. DR rats. TheDR rats had significantly greater AP and α-glucosidase activityand more pronounced suppression of energy intake than obeseDIO rats. It indicates that the increase of enzyme activities andthe lowered effectiveness of nutrient absorption might be asignificant factor preventing the expression of obesity proneness.This information contributes to a better understanding ofa complex interaction between HF diet feeding and smallintestinal adaptability, which determines the energy homeostasisand predict the ability to resist or develop obesity in thesephenotypes.