Open AccessAdvanced glycation end-product pentosidine accumulates in various tissues of rats with high fructose intake
Author(s) -
Kateřina Mikulı́ková,
Adam Eckhardt,
Jaroslav Kuneš,
Josef Zicha,
Ivan Mikšı́k
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931093
Subject(s) - pentosidine , glycation , advanced glycation end product , chemistry , catabolism , elastin , fructose , maillard reaction , biochemistry , extracellular matrix , medicine , endocrinology , enzyme , biology , receptor , genetics
The slowly metabolized proteins of the extracellular matrix,typically collagen and elastin, accumulate reactive metabolitesthrough uncontrolled non-enzymatic reactions such as glycationor the products arising from the reaction of unsaturated longchain fatty acid metabolites (possessing aldehydic groups). Atypical example of these non-enzymatic changes is the formationof advanced glycation end-products (AGEs), resulting from thereaction of carbohydrates with the free amino group of proteins.The accumulation of AGEs and the resulting structural alterationscause altered tissue properties (increased stiffness, reducedelasticity) that contribute to their reduced catabolism and to theiraging. Posttranslational nonenzymatic modifications of theproteins of the extracellular matrix (the formation of a typicalAGE product - pentosidine) were studied in three types of tissueof three rat strains subjected to a high-fructose diet. Chronic(three-week) hyperglycemia (resulting from fructose loading)caused a significant increase in pentosidine concentration mainlyin the aorta and skin of the three rat strains (Lewis, Wistar andhereditary hypertriglyceridemic rats).