Open AccessChanges of extracellular matrix of rat cornea after exposure to hypoxia
Author(s) -
Gabriela Mahelková,
J Korynta,
A Moravová,
Jaovotná,
Richard Vytášek,
J. Wilhelm
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931087
Subject(s) - cornea , zymography , hypoxia (environmental) , chemistry , extracellular matrix , nitrotyrosine , matrix metalloproteinase , pathology , biochemistry , enzyme , oxygen , ophthalmology , medicine , nitric oxide synthase , organic chemistry
The purpose of the study was to check whether hypoxia ofcorneal tissue increases the collagenolytic activity due to releaseof reactive oxygen and nitrogen species. Rats were exposed tohypoxia 10 % O2 for 4, 14, and 21 days. The radical tissue injurywas measured by the level of nitrotyrosine and changes in thelipoperoxide-related fluorophores. Collagen protein compositionwas analyzed by slab gel electrophoresis. The activity ofgelatinolytic enzymes was studied using the zymography. Thevascularization of the corneas was measured. We found nodifferences in the corneal tissue in the gel electrophoretic profileof collagenous proteins and gelatinolytic activity betweennormoxic and hypoxic rats. We did not find any sign of radicaltissue injury. There were no changes in the vascularization ofcorneas after exposition to hypoxia. The environmental 10 %hypoxia does not induce radical tissue injury and an increase ofcollagenolytic activity in the rat cornea.