Open AccessExpression of phospho-Elk-1 in rat gut after the whole body γ-irradiation
Author(s) -
Daniel Driák,
Jan Österreicher,
Zuzana Řeháková,
Zdeňka Vilasová,
Jiřina Vávrová
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931028
Subject(s) - jejunum , enterocolitis , irradiation , gastrointestinal tract , ionizing radiation , enteritis , biology , pathology , medicine , andrology , physics , nuclear physics
Gastrointestinal form is the second stage of acute radiationsyndrome (ARS) with a threshold dose of 8 Gy in man. Itrepresents an absolutely lethal clinical-pathological unit, necrohemorrhagic enteritis and proctocolitis, with unknown causaltherapy. Elk-1 is a protein acting as a transcription factoractivating specified genes. The purpose of our study was toexamine the expression of phospho-Elk-1 in irradiated jejunumand transversal colon of rats with radiation-induced enterocolitisand to assess the importance of this transcriptional factor as abiodosimetric marker of radiation-induced enteropathy. Thelaboratory rats were randomly divided into 21 groups, 10 animalsper group, and irradiated with whole body γ-irradiation of 1, 5,10, 15, and 20 Gy. Samples of jejunum and transversal colonwere taken 24, 48, 72, and 96 hours later, immunohistochemically stained, and the phospho-Elk-1 expression wasexamined using computer image analysis. A group of 10 shamirradiated animals was used as control. Significantly increasedexpression of phospho-Elk-1 in rat jejunum has been found in alltime intervals after irradiation by sublethal doses of 1 and 5 Gy,whereas after the irradiation by lethal doses, the expression ofphospho-Elk-1 in rat jejunum varied considerably. Significantlyincreased expression of phospho-Elk-1 in transversal colon hasalso been found in the first days after irradiation by sublethaldoses of 1 and 5 Gy. After irradiation by lethal doses, tere was nouniform pattern of the changes in the expression of phospho-Elk1 in rat transversal colon. The detection of phospho-Elk-1 mightbe considered as a suitable and very sensitive biodosimetricmarker of radiation-induced injury of small and large intestine.According to our knowledge, this is the first study on thephospho-Elk-1 expression in irradiated jejunum and transversalcolon in the rat.