Open AccessInvolvement of nitric oxide in noradrenaline-induced changes in the activity of antioxidant enzymes and lipid peroxidation in rat brown adipose tissue and heart
Author(s) -
Antonija Perović,
Tamara Vučković,
Gordana Cvijić,
Jelena Djordjević,
Vesna Davidović
Publication year - 2008
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931026
Subject(s) - nitric oxide , superoxide dismutase , chemistry , brown adipose tissue , medicine , endocrinology , lipid peroxidation , dismutase , catalase , antioxidant , nitric oxide synthase , biochemistry , adipose tissue , biology
The effect of exogenous noradrenaline (NA) (1.6 mg.kg-1 i.p.,35 min prior sacrifice) on the activity of antioxidant enzymes(AOE) copper zinc superoxide dismutase (CuZnSOD), manganesesuperoxide dismutase (MnSOD) and catalase (CAT), as well aslipid peroxides (LP) concentration were studied in the ratinterscapular brown adipose tissue (IBAT) and heart of saline(controls) and Nω-nitro-L-arginine methyl ester (L-NAME) treatedrats (10 mg.kg-1, i.p., during 3 days and 20 min before NA). NAdifferently affects both AOE activities and LP production in theIBAT and heart. Thus, NA inhibited the activity of all IBAT AOEand LP production while in the heart it markedly increased CATactivity only, but had no effect on any of SODs activities and LPconcentration. L-NAME, a nitric oxide synthase blocker,completely abolished the NA-induced inhibition of the IBAT AOEand LP production, whereas in the heart it was without effect. Inconclusion, these results indicate that both NA and L-NAMEeffects on AOE activity and LP production are tissue specific andalso suggest that nitric oxide mediates the NA-induced inhibitionof AOE activity and LP production in the IBAT only.