Open AccessAdenosine potentiates stimulatory effects on granulocyte-macrophage hematopoietic progenitor cells in vitro of IL-3 and SCF, but not those of G-CSF, GM-CSF and IL-11
Author(s) -
Milan Höfer,
A Vácek,
M. Pospı́šil,
Lenka Weiterová,
Jarmila Holá,
Denisa Štreitová,
Vladimı́r Znojil
Publication year - 2006
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.930854
Subject(s) - haematopoiesis , interleukin 3 , granulocyte macrophage colony stimulating factor , adenosine , granulopoiesis , progenitor cell , stem cell factor , hematopoietic growth factor , biology , growth factor , cytokine , interleukin , macrophage , stem cell , granulocyte , endocrinology , medicine , in vitro , immunology , microbiology and biotechnology , biochemistry , cytotoxic t cell , interleukin 21 , receptor
The aim of the studies was to ascertain if adenosine is able to co-operate with selected hematopoietic growth factors and cytokines, namely with granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), interleukin-3 (IL-3), and interleukin-11 (IL-11), in inducing the growth of colonies from hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC) from normal bone marrow cells in vitro. Adenosine was found not to produce any colonies when present in the cultures as the only potential stimulator. All the tested cytokines and growth factors were observed to induce the growth of distinct numbers of GM-CFC colonies, with the exception of IL-11. When suboptimal concentrations of the evaluated cytokines and growth factors were tested in the cultures in which various concentrations of adenosine were concomitantly present, mutually potentiating effects were found in the case of IL-3 and SCF. These results confirm the role of adenosine in regulation of granulopoiesis and predict IL-3 and SCF as candidates for further in vivo studies of their combined administration with adenosine.