Open AccessUJI TOKSIK SUSPENSI SEL PUNCA CD34+ MANUSIA PADA TIKUS SPRAGUE DAWLEY (SD)
Author(s) -
Basuki Supartono
Publication year - 2019
Publication title -
jurnal profesi medika
Language(s) - English
Resource type - Journals
eISSN - 2621-1122
pISSN - 0216-3438
DOI - 10.33533/jpm.v8i2.679
Subject(s) - cd34 , cord blood , haematopoiesis , stem cell , toxicity , umbilical cord , pharmacology , kidney , chemistry , medicine , andrology , immunology , biology , microbiology and biotechnology
: Research of CD34+ cells intervention to rats is not yet done. This researchis the latest research which investigated the toxic reaction of CD34+ hematopoietic stem cell intervention in non engineered rats.Methods : 3 Sprague Dawley rats, male, 7 months, 285+18,5 g. Rat 1 : received intraartricular injection of human peripheral blood CD34+ cells (105 cells). Rat 2 : received intraartricular injection of human cord blood CD34+ cells (105 cells). Rat 3 : received intraartricular injection of human cord blood CD34+ cells (106 cells), hyaluronic acid and grown factors. All rats were examined hematological, blood chemistry parameters and microscopic of the liver and kidney.Result : All rats showed good condition. There is slight increasing parameter in hematological and blood chemistry but liver and kidney in normal microscopic condition. No hiperacute rejection reaction : edema, systemic bleeding. There is no hyper acute and acute reaction.Conclusion : Human CD34+ cells suspension with or without scaffold and growth factors does not cause toxicity reaction in naïve Sprague Dawley rats.