Open AccessA Non-Frozen Living Tissue Bank for Allotransplantation Using Green Tea Polyphenols
Author(s) -
Suong Hyu Hyon
Publication year - 2004
Publication title -
yonsei medical journal/yonsei medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.702
H-Index - 63
eISSN - 1976-2437
pISSN - 0513-5796
DOI - 10.3349/ymj.2004.45.6.1025
Subject(s) - cryopreservation , allotransplantation , polyphenol , chemistry , andrology , tissue bank , living cell , biology , microbiology and biotechnology , transplantation , surgery , medicine , biochemistry , embryo , bioinformatics , antioxidant
Generally, mammalian cells and living tissues can be cryopreserved in a frozen state at very low temperatures over a long storage term. The survival rate of cell suspensions is often acceptable however, living tissues suffer a variety of injuries. In this paper, it was demonstrated that the addition of polyphenols extracted from green tea to conventional cell culture medium and tissue compatible liquid, can control cell proliferation and also preserve tissues for several months at ordinary room temperature, including such tissues as blood vessels, cartilage, islet cells and corneas. This protocol allows a non-frozen living tissue bank to be established using the preservation fluid described.