Open AccessStructure and Function of a Minimal Receptor Activation Domain of Parathyroid Hormone
Author(s) -
Eun Jin Lee,
Hai Young Kim,
Min Kyu Cho,
Weontae Lee,
Sung Kil Lim
Publication year - 2004
Publication title -
yonsei medical journal/yonsei medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.702
H-Index - 63
eISSN - 1976-2437
pISSN - 0513-5796
DOI - 10.3349/ymj.2004.45.3.419
Subject(s) - chemistry , residue (chemistry) , stereochemistry , oligopeptide , peptide , receptor , protein structure , amino acid , parathyroid hormone , crystallography , calcium , biochemistry , organic chemistry
The structure and function of short-length amino terminal PTH analogues were studied. The substitution of Leu(7) with Phe in [Ala(3,10)Leu(7)Arg(11)]rPTH(1-11)NH(2) analogue peptides did not show any reduction in cAMP formation. Replacement of the 1st, 7th and 8th residues revealed different activities, depending upon the residue type. The substitution of Ala1 by Ser in [Ala(3,10)Leu(7)Arg(11)]rPTH(1-11)NH(2) caused nearly a complete loss of cAMP formation. Meanwhile, NMR analysis of [(Ala(1)/ Ser(1))Ala(3,10)(Leu(7)/Phe(7))Arg(11)]rPTH(1-11)NH(2) revealed an alpha-helical backbone structure with a flexible conformation at the carboxyl-terminus. The overall results suggest that 11-residue short oligopeptide analogues of PTH tend to form an alpha-helical structure and the different activities of those analogues could be associated with residue specificity rather than the secondary conformational structure.