Open AccessBehavior of Fibroblasts on a Porous Hyaluronic Acid Incorporated Collagen Matrix
Author(s) -
Hwal Suh,
Jong Eun Lee
Publication year - 2002
Publication title -
yonsei medical journal/yonsei medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.702
H-Index - 63
eISSN - 1976-2437
pISSN - 0513-5796
DOI - 10.3349/ymj.2002.43.2.193
Subject(s) - hyaluronic acid , collagenase , fibroblast , matrix (chemical analysis) , extracellular matrix , type i collagen , biophysics , chemistry , ultimate tensile strength , dermal fibroblast , materials science , biomedical engineering , in vitro , composite material , biochemistry , anatomy , pathology , enzyme , biology , medicine
A hyaluronic acid (HA) incorporated porous collagen matrix was fabricated at -70 degrees C by lyophilization. The HA incorporated collagen matrix showed increased pore size in comparison with collagen matrix. Biodegradability and mechanical properties of matrices were controllable by varying the ultraviolet (UV) irradiation time for cross-linking collagen molecules. Addition of HA to collagen matrix did not effect ultimate tensile stress after UV irradiation. HA incorporated collagen matrices demonstrated a higher resistance against the collagenase degradation than collagen matrix. In an in vitro investigation of cellular behavior using dermal fibroblasts on the porous matrix, HA incorporated collagen matrix induced increased dermal fibroblast migration and proliferation in comparison with collagen matrix. These results suggest that the HA incorporated collagen porous matrix assumes to enhance dermal fibroblast adaptation and regenerative potential.