Open AccessSeroreactivities of proteinases ofCandida albicans, C. tropicalis, andC. parapsilosisin sera from variousCandidaspecies-infected mice
Author(s) -
Kyung Ho Lee,
Wan Seon Shin,
Hyun Sook Park,
Joo Young Park,
Choon Myung Koh
Publication year - 1997
Publication title -
yonsei medical journal/yonsei medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.702
H-Index - 63
eISSN - 1976-2437
pISSN - 0513-5796
DOI - 10.3349/ymj.1997.38.3.178
Subject(s) - candida parapsilosis , microbiology and biotechnology , antiserum , biology , heterologous , candida albicans , candida tropicalis , corpus albicans , candida krusei , antibody , biochemistry , immunology , gene
From the culture filtrates of C. albicans, C. tropicalis and C. parapsilosis, proteinases were purified using a series of chromatographic steps consisting of DEAE-Sepharose, Sephacryl S-200 and size-exclusion HPLC which removed contaminating mannoproteins and extraneous proteins. Anti-Candida proteinase antibodies in sera from mice infected with various Candida species were detected using ELISA for serodiagnosis of candidiasis. Three proteinases were blotted by homologous and heterologous anti-proteinase antisera on Western blot analysis. All sera from six Candida species-infected mice were reactive with proteinases of C. albicans, C. tropicalis, and C. parapsilosis, although C. glabrata, C. guilliermondii, and C. krusei did not secrete proteinase. The seroreactivities of proteinase with sera from mice infected with homologous C. albicans and C. tropicalis were higher than those with sera from heterologous Candida species-infected mice. These results suggest that three proteinases have at least one common epitope, but its application for diagnosis of candidiasis should be considered with limits of specificity.