z-logo
open-access-imgOpen Access
Triton X-100 induces apoptosis in human hepatoma cell lines
Author(s) -
Ju Mi Ahn,
Se Jong Kim,
Hoguen Kim,
ChanIl Park,
Won Ho Kim,
Jeon Han Park
Publication year - 1997
Publication title -
yonsei medical journal/yonsei medical journal
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.702
H-Index - 63
eISSN - 1976-2437
pISSN - 0513-5796
DOI - 10.3349/ymj.1997.38.1.52
Subject(s) - tunel assay , apoptosis , dna fragmentation , microbiology and biotechnology , in situ nick end labeling , terminal deoxynucleotidyl transferase , fragmentation (computing) , chemistry , cell culture , programmed cell death , dna , lactate dehydrogenase , biology , biochemistry , enzyme , ecology , genetics
The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here