Open AccessTriton X-100 induces apoptosis in human hepatoma cell lines
Author(s) -
Ju Mi Ahn,
Se Jong Kim,
Hoguen Kim,
ChanIl Park,
Won Ho Kim,
Jeon Han Park
Publication year - 1997
Publication title -
yonsei medical journal/yonsei medical journal
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.702
H-Index - 63
eISSN - 1976-2437
pISSN - 0513-5796
DOI - 10.3349/ymj.1997.38.1.52
Subject(s) - tunel assay , apoptosis , dna fragmentation , microbiology and biotechnology , in situ nick end labeling , terminal deoxynucleotidyl transferase , fragmentation (computing) , chemistry , cell culture , programmed cell death , dna , lactate dehydrogenase , biology , biochemistry , enzyme , ecology , genetics
The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.