Open AccessStudies on effects of different solvent extracted samples of O. sanctum (Basil) stem on the growth of S. typhi
Author(s) -
Smita Kumari,
Vinod Prasad
Publication year - 2020
Publication title -
flora and fauna/flora and fauna
Language(s) - English
Resource type - Journals
eISSN - 2456-9364
pISSN - 0971-6920
DOI - 10.33451/florafauna.v26i2pp209-212
Subject(s) - salmonella typhi , ethyl acetate , agar diffusion test , solvent , typhoid fever , antibacterial activity , chemistry , methanol , agar , traditional medicine , chromatography , biology , biochemistry , bacteria , medicine , microbiology and biotechnology , organic chemistry , escherichia coli , genetics , gene
Typhoid is a problematic fever, caused by Salmonella typhi. Present investigation is based upon studies of antibacterial effects of different solvent extracted samples of O. sanctum stem on growth of S. typhi. The crude extracts of stem of O. sanctumwere prepared from three different solvents such as methanol, ethyl acetate and hot water. Four different concentrations (25%, 50%, 75% and100%) were prepared from each one of the solvent extracted sample. Agar well diffusion method was used totest the antibacterial activity of different solvent extracted samples of O. sanctum stem. Analysis of data indicates that a metabolic extract of O. sanctum stem shows maximum zone of inhibition and it was of 26mm at 100% concentration and minimum zone of inhibition was of 19mm at 50% concentration. Ethyl acetate extract of O. sanctum stem shows maximum zone of inhibition and it was of 29mm at 100% concentration and minimum was of 14mm at 25% concentration. Hot water extract of O. sanctum stem shows maximum zone of inhibition and it was of 28mm at 100% concentration and minimum zone of inhibition was of 12mm at 25% concentration. This study indicates that ethyl acetate extract of O. sanctum stem is more effective against S. typhi as compared to methanol and hot water extracted solvent. Contrary to the antibiotics or synthetic medicine associated with many side effects, this plant part can be used as a herbal medicines against Salmonella typhi infection.