Open AccessProduction of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
Author(s) -
Raquel Freitas,
Juliane Döering Gasparin Carvalho,
Laura Maria Bruno,
Gustavo Adolfo Saavedra Pinto
Publication year - 2020
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v9i7.4280
Subject(s) - industrial fermentation , lactobacillus rhamnosus , aeration , fermentation , biomass (ecology) , food science , substrate (aquarium) , pulp and paper industry , chemistry , lactobacillus , biology , agronomy , engineering , ecology , organic chemistry
The objective of this work was to produce Lactobacillus rhamnosus BRM 029693 biomass in a fermenter with the aim to expand its production scale. Lactobacillus rhamnosus BRM 029693 was grown in a fermenter under different aeration conditions (in surface and depth) using a commercial substrate (MRS broth) and simplified substrate formulated in the laboratory itself, and under different pH conditions (4.7, 5.2, 5.7). The highest biomass production on a fermenter scale occurred using synthetic MRS, 1 v.v.m. aeration on the medium surface and without pH control (3.2 g/L) among all the tests. The biomass production was only 0.7 g/L when the MRS medium was replaced by simplified MRS. The pH also influenced the biomass production, reaching 0.9 g/L when the fermentation pH was controlled at pH 4.7.