Open AccessFactors affecting the cryopreservation of oocytes and embryos in buffalo (Bubalus bubalis): A review
Author(s) -
Satish Kumar,
Maiana Silva Chaves,
Ana Flávia Bezerra Silva,
W. G. Vale,
Natanael Aguiar Braga Negreiros,
Irving Mitchell Laines Arcce,
Luciana Magalhães Melo,
Vicente José de Figueirêdo Freitas
Publication year - 2022
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v11i4.27337
Subject(s) - cryopreservation , vitrification , andrology , embryo cryopreservation , embryo , bubalus , biology , reproductive technology , microbiology and biotechnology , medicine , ecology
Cryopreservation of oocytes and embryos is a supplementary technique for in vivo and in vitro embryo production. In the last decade, the technique has been improved but did not achieve a satisfactory commercial level. Also, the results were variable. The pregnancy and live birth rates were 16 to 52% and 5.3 to 26.9% after implantation of vitrified embryos, respectively (from the year 1993 to 2020). In final consideration, the quality of oocytes and embryos is the most important factor to obtain better results after cryopreservation. The addition of antioxidant and lipid-depleting agents to the medium is a factor that improves cryogenic tolerance. Additionally, two-step vitrification is a reliable method for cryopreservation. Studying epigenetic effects and introducing lipidomic, transcriptomic and proteomic technologies can help improve this technology.