Open AccessXanthan gum and sodium hypochlorite in vitro rooting of Gerbera hybrida cv. Essandre
Author(s) -
Brenda Lima Ribeiro,
J. F. Ribeiro,
Sílvio Lopes Teixeira,
Antônio Bruno Nunes Oliveira,
Ana Rosa Peixoto,
Anna Christina Passos Menezes,
J. C. de Souza,
Cristiane Domingos da Paz
Publication year - 2022
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v11i4.25143
Subject(s) - xanthan gum , sterilization (economics) , sodium hypochlorite , agar , chemistry , sodium alginate , hypochlorite , food science , polysaccharide , gerbera , horticulture , sodium , biology , biochemistry , bacteria , materials science , organic chemistry , rheology , monetary economics , economics , composite material , foreign exchange market , genetics , foreign exchange
The aim of this study was to check the use of the following gelling agents: the xanthan gum “Adicel®” and the stabilizer “Super Liga Neutra®” to replace agar in the in vitro rooting phase of Gerbera hybrida cv. Essandre. Additionally, the possibility of using chemical sterilization of both culture media and glassware with sodium hypochlorite to replace autoclaving was analyzed. The gelling agents, xanthan gum “Adicel®” and the stabilizer “Super Liga Neutra®” were tested at the following concentrations (g L-1): 7; 9; 11; 13; 15; 17; 19; and 21. No concentrations of Super Liga Neutra® provided effective solidification. Concentrations of 17 and 21 g of Adicel® provided a good gelling of the culture medium, which was compared to the medium containing agar (control) with two types of sterilization: autoclaving (for 20 and 40 minutes) and chemical sterilization. Autoclaving for 20 minutes did not provide effective elimination of contamination in the culture medium containing xanthan gum; this only occurred when autoclaving time increased to 40 minutes. Plant development in culture media containing 17 and 21 g of xanthan gum, either sterilized by autoclaving for 40 minutes or at a concentration of 17 g xanthan gum using sodium hypochlorite, was statistically the same as the control that contained agar. However, plant development at a concentration of 21g of xanthan gum in a sterilized medium using sodium hypochlorite was lower than that observed in media containing agar.