Open AccessEffects of tobacco smoke inhalation on the morphology and cell proliferation rate in Wistar rats submandibular glands
Author(s) -
Lorraine Stephanie Hiss,
Anna Cecília Dias Maciel Carneiro,
Rodrigo César Rosa,
Adilha Misson Rua Micheletti,
José Batista Volpon,
Virgínia Oliveira Crema
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i8.17327
Subject(s) - cotinine , analysis of variance , inhalation , saliva , smoke inhalation , cigarette smoke , submandibular gland , nicotine , medicine , tobacco smoke , immunohistochemistry , parenchyma , endocrinology , chemistry , andrology , pathology , biology , toxicology , anatomy , polymer chemistry
Cigarette smoke compromises the salivary glands, altering saliva. Cotinine is the most effective biomarker to assess tobacco consumption, and passive smokers are characterized by serum cotinine levels above 2.1 ng/mL. This study aimed to evaluate the effect of exposure to cigarette smoke on rats submandibular glands. The morphology, the proliferative cells of intercalated ducts (immunohistochemistry for KI-67) and its correlation with serum cotinine levels were analyzed. Wistar rats were grouped: control (C); inhalation of smoke for 30 days (T1) and 60 days (T2). Data were analyzed with the Levene variance homogeneity test, Spearman's test, ANOVA and Tukey's post-test, with significance p<0.05. Morphology showed a preserved parenchyma in C, and altered secretory cells in T1 and T2. There was a significant reduction in the number of proliferative cells [ANOVA, F = 4.726; d.f. = 2, 26; p<0.05]: C vsT1 (p<0.05) and C vs T2 (p<0.05). T1 and T2 serum cotinine levels were significantly higher (p<0.05) than C. There was a significant negative correlation [ρ = -0.418, n=27, p<0.05] between serum cotinine levels and the rate of cellular proliferation. These results suggest that of tobacco smoke inhalation has a decreased of the cellular proliferation rate and altered morphology in submandibular glands de ratos Wistar.