Open AccessComparative effectiveness of Metarhizium rileyi, novaluron, and glyphosate on immune system, development, and redox metabolism of Anticarsia gemmatalis
Author(s) -
Ana Paula Vargas Visentin,
Lúcia Rosane Bertholdo,
Rahyssa Chagas Hahn,
Rafaela Andressa Thomazoni,
Luciana Bavaresco Andrade,
Cátia Santos Branco,
Mirian Salvador,
Neiva Monteiro de Barros
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i6.15611
Subject(s) - anticarsia gemmatalis , biology , novaluron , entomopathogenic fungus , caterpillar , toxicology , diflubenzuron , biological pest control , pesticide , botany , larva , ecology , beauveria bassiana
Anticarsia gemmatalis is one of the most important pests in world soybean crop. The most common intervention is the application of agrochemicals, such as novaluron and glyphosate. Among biological control agents, much attention has been drawn to entomopathogenic fungi, as Metarhizium rileyi. Here, we examined the changes that occur in the immune system (total and differential hemocyte count), secondary effects (caterpillar morphology), and oxidative metabolism after the caterpillars were exposed to M. rileyi, novaluron or glyphosate. M. rileyi was able to induce changes in the width, length, and weight of A. gemmatalis pupae, along with an increased in the number of defense cells. Novaluron prompt changes the insect’s immunity, and glyphosate caused milder immunological effects. However, it caused significant secondary effects including malformations in pupae and adults, and an increase in nitric oxide (NO) levels. Mortality observed when treating insects with novaluron and malformations due to glyphosate treatments did not occur due to oxidative stress. However, when insects were exposed to M. rileyi, we verified significantly increased levels of NO and concluded that these insects died due to oxidative stress. Our data provide evidence that contributes to better understanding the mechanism of herbicide-fungus interaction in the management of Anticarsia gemmatalis.