Open AccessCytotoxicity and osteogenic potential of experimental medication with calcium hydroxide and activated charcoal
Author(s) -
Gabriela Sumie Yaguinum Gonçalves,
Danielle Gregório,
Isabelly Ribeiro Custódio,
Luciana Prado Maia,
Bruno Piazza,
Graziela Garrido Mori
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i5.14671
Subject(s) - cytotoxicity , calcium hydroxide , alkaline phosphatase , activated charcoal , chemistry , mineralization (soil science) , nuclear chemistry , calcium , charcoal , mtt assay , in vitro , sodium hydroxide , hydroxide , biochemistry , organic chemistry , enzyme , adsorption , nitrogen
Objectives: The aim of this study was to evaluate the cytotoxicity and osteogenic potential of experimental medications containing Calcium Hydroxide (CH) and Activated charcoal (AC). Methodology: Osteoblastic (MC3T3) and fibroblastic (L929) cells were cultivated in 96 well plates (1 x 104 cells/well) and, after 24 h, treated with extracts, according to experimental groups [(Experimental groups: C - Control; CH - CH paste; CH+10%AC - experimental paste 1 (CH paste + 10% AC); CH+5%AC - experimental paste 2 (CH paste + 5% AC)]. Cytotoxicity and potential osteogenic was performed by MTT and alkaline phosphatase activity, respectively, after 1, 3 and 7 days. Results: For intergroups comparisons, ANOVA 2 factors were used, followed by Tukey test (p 0.05). For osteogenic potential, it was found that all experimental groups stimulated mineralization in relation to control group, except for experimental paste 2 at 7 days. Conclusion: The addition of AC to the CH paste does not modify toxicity and osteogenic properties. Add 10% of AC appears to be more effective than 5%.