Open AccessSciatic nerve-conditioned medium with the addition of Croton blanchetianus Baill essential oil promotes morphological plasticity in spinal cord cultured cells
Author(s) -
Luciana Cristina Borges Fernandes,
Salvador Viana Gomes,
Ianara Mendonça da Costa,
Francisca Idalieta,
Rodrigo Freire Oliveira,
Lucídio Clebeson de Oliveira,
Eduardo Pereira de Azevedo,
Francisco Fábio Mesquita Oliveira,
Cynthia Cavalcanti de Albuquerque,
Francisco Felipe Maia da Silva,
Jael Soares Batista,
José Rodolfo Lopes de Paiva Cavalcanti,
Carlos Iberê Alves Freitas,
Fausto Pierdoná Gúzen
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i5.14591
Subject(s) - neun , neuroprotection , spinal cord , neuroscience , spinal cord injury , regeneration (biology) , neurogenesis , biology , microbiology and biotechnology , medicine , pathology , immunohistochemistry
Spinal cord injury is a devastating condition that leads to significant disabilities. The treatment for this problem is a challenge in neuroscience, and it is necessary to combine different strategies to obtain functional recovery outcomes. There are many herbal natural products, such as Croton blanchetianus Baill (CB) essential oil, a Brazilian semi-arid bush with neuroprotective substances tested in regeneration processes and synaptic plasticity. Thus, this study analyzed the cellular plasticity of spinal cord neurons and glial cells in the presence of sciatic nerve-conditioned medium (SNCM) before the addition of CB essential oil. Cell morphology was assessed over 96 hours, and immunocytochemistry analyses were conducted for GFAP, GAP-43 and NeuN. Photomicrographs were made by scanning electron microscopy (SEM). Morphological analysis showed evident trophic development in the groups that received CB essential oil (P>0.000), immunoreactivity for GFAP, GAP-43 and NeuN and the plasticity of these cells were confirmed by SEM. This pioneer study about the plasticity of spinal cord neurons and glial cells opens new possibilities and techniques with essential oils for cell therapy in the presence of SNCM, which promoted neuroprotective action.