Open AccessIn vitro maturation of domestic cat oocytes subjected to different incubation times
Author(s) -
Denilsa Pires Fernandes,
Fernanda Araújo dos Santos,
Luã Barbalho de Macêdo,
Roberta Izzo,
Muriel Magda Lustosa Pimentel,
Alexsandra Fernandes Pereira,
Marcelo Barbosa Bezerra
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i3.13074
Subject(s) - incubation , in vitro maturation , andrology , in vitro , polar body , biology , oocyte , incubation period , zoology , embryo , medicine , biochemistry , microbiology and biotechnology
The aim of this study was to evaluate the effect of three different incubation times on in vitro maturation of domestic cat oocytes. Thus, ovaries (n = 42) were submitted to slicing procedure and the oocytes recovered were classified; only good quality oocytes (Grade I and II) underwent in vitro maturation for three different periods (24 vs. 30 vs. 36 h) in supplemented TCM-99 medium. After, oocytes were evaluated for cumulus cell expansion and presence of the first polar body. After six replicates (7 ± 1,7 ovaries per replicate), a total of 334 viable oocytes were recovered. Differences (p <0.05) were observed regarding the percentage of oocytes presenting expansion of the cumulus cells, where higher values were observed in the group of oocytes incubated for 36 h (84.3%), when compared to 30 (73.4%) and 24 h (71.0%). Moreover, differences were also observed regarding the presence of the first polar body (24 h: 29.7%; 30 h: 58.2%; 36 h: 69.8%). We conclude that the incubation period influenced the maturation rates, indicating 36 h as the ideal period for the in vitro maturation of domestic cat oocytes in supplemented TCM-199 medium.