Open AccessChemical profile, bactericidal in vitro potential and toxicity against Artemia salina Leach of essential oils obtained from natural condiments
Author(s) -
Thércia Gabrielle Teixeira Martins,
Paulo Victor Serra Rosa,
Ari Pereira de Araújo Neto,
Ana Maria Almeida Silva Carvalho,
Leila da Silva Silveira,
Ione Rocha Neves,
Carlos Eduardo Pereira Conceição,
Francilidia Oliveira Vitorino de Assunção Conceição,
Ricardo Teixeira de Sousa,
Paulo Victor Serra Rosa,
Gustavo Oliveira Everton,
Victor Elias Mouchrek Filho
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i2.12898
Subject(s) - artemia salina , bacillus cereus , bioassay , antimicrobial , food science , context (archaeology) , essential oil , antibacterial activity , biology , toxicity , chemistry , bacteria , microbiology and biotechnology , paleontology , organic chemistry , genetics
Interest in essential oils and their food applications has grown due to the negative reaction of consumers to synthetic chemical additives intentionally added in industrialized products in order to increase their shelf life. In this context, the present study aimed to evaluate the chemical profile, toxicity to Artemia salina Leach and antibacterial activity in vitro of essential oils obtained from natural condiments on bacteria of clinical and food importance. Plant material was obtained in the municipality of São Luís-MA. The essential oils were extracted by hydrodistillation at 100 °C/3 h. Folin Ciocalteau methodology was performed for the determination of total phenolics. The toxicity assay was performed using the artemia salina Leach lethality bioassay. Antimicrobial activity followed the methodology described by the Clinical and Laboratory Standards Institute using the Broth Disc Diffusion and Dilution Method for the action of essential oils against the bacteria Escherichia coli, Salmonella sp., Staphylococcus aureus and Bacillus cereus. The presence of bioactive classes in the plant materials used in this research was identified and the essential oils obtained were classified as nontoxic in the toxicity assay, presenting low lethality to the micro crustacean Artemia salina Leach. In the bactericidal activity assay, the essential oils of O. vulgare, T. vulgaris, C. zeylanicum showed significant results, classified as efficient against the microorganisms tested. Finally, the use of essential oils classified as active and nontoxic is highlighted in this study as alternatives in the control and combat of pathogenic microorganisms presenting a proposal of natural product with low cost of obtaining and high market potential.