Open AccessAnálise de proteínas salivares de corredores de rua após evento esportivo real
Author(s) -
Cícero Matheus Lima Amaral,
Iago Almeida da Ponte,
Géssica de Souza Martins,
Daniel Freire Lima,
Abelardo Barbosa Moreira Lima Neto,
Ana Cristina de Oliveira Monteiro-Moreira,
Maria Izabel Florindo Guedes
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i10.18183
Subject(s) - saliva , proteome , cystatin , microbiology and biotechnology , albumin , western blot , chemistry , alpha (finance) , cystatin c , medicine , biology , biochemistry , surgery , gene , renal function , construct validity , patient satisfaction
The aim was to characterize acute exercise-induced changes in salivary proteins of street runners. Saliva samples from 12 adult male athletes were collected before and immediately after a street race. Two groups were formed based on the distance covered, 5 km (n=4) and 10 km (n=8). Samples were subjected to depletion of amylase, albumin and immunoglobulin G. Then they were concentrated, digested and analyzed by nano-UPLC-tandem nano-ESI-MSE. A total of 69 proteins were identified. Significant changes were observed in the expression of 15 proteins in the 5 km group and 13 proteins in the 10 km group. Among the proteins with altered expression, only 7 had already been described in the literature in similar models (Alpha-Amylase 1, Lactoperoxidase, Alpha Skeletal Muscle Actin, Cystatin-B, Cystatin- SN, Cystatin- SA and Androgen-regulated Protein 3B in the submaxillary gland). This study has shown that street running induces acute changes in the salivary proteome. The results obtained add to the limited data available in the literature in the search for a better understanding of the acute effects of exercise.