Open AccessVasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade
Author(s) -
Joelmir Lucena Veiga da Silva,
Fabiana de Andrade Cavalcante,
Tânia Maria Sarmento da Silva,
Alessandro Elery Ramos,
Thárcia Kiara Beserra de Oliveira,
Schirley Cristina Almeida Pereira,
Bagnólia Araújo da Silva
Publication year - 2021
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v10i1.11845
Subject(s) - chemistry , aorta , phenylephrine , contraction (grammar) , endothelium , blockade , l type calcium channel , pharmacology , calcium , anesthesia , chromatography , voltage dependent calcium channel , medicine , biochemistry , receptor , organic chemistry , blood pressure
Previous results showed the ethyl acetate phase (SP-AcOEt), obtained from aerial parts of Solanum paludosum, relaxed the aorta isolated in the endothelium-dependent and -independent manner. The vasorelaxant effects of SP-AcOEt was not characterized on aorta rings endothelium-denuded, thus this work aimed to elucidate the mechanisms endothelium-independent vasorelaxation on rat isolated aorta. The aorta was isolated from Wistar rats and mounted in glass baths containing 6 mL of normal Krebs physiological solution with pH at 7.4. The preparation was maintained at 37ºC and bubbled continuously with a mixture of 95% O2 and 5% CO2. Aortic rings were maintained for 1 hour by a resting tension of 1g and next were contracted with phenylephrine after the sustained contraction ACh was added to access the integrity of the endothelium. SP-AcOEt relaxed pre-contracted aorta by KCl-30mM or -80mM in a similar manner, suggesting blockade CaV, but not channel-K+ participating. SP-AcOEt also inhibited the contraction induced by CaCl2 and relaxed pre-contracted aorta by (±)-BayK8644 (EC50 = 16.9±1.3 μg/mL), which confirms the involvement of L-type CaV blockade. SP-AcOEt presented vasorelaxation endothelium-independent that involves L-type CaV blockade.