Open AccessValidation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L.
Author(s) -
А. Е. Суханов,
А. Н. Ставрианиди,
Kubasova E.D.,
Andrii Panasiuk,
О. В. Буюклинская
Publication year - 2020
Publication title -
razrabotka i registraciâ lekarstvennyh sredstv
Language(s) - English
Resource type - Journals
eISSN - 2658-5049
pISSN - 2305-2066
DOI - 10.33380/2305-2066-2020-9-4-107-114
Subject(s) - diosgenin , trigonella , sapogenin , chromatography , high performance liquid chromatography , chemistry , raw material , traditional medicine , botany , biology , organic chemistry , medicine , alternative medicine , pathology
. Modern pharmacognostic research is aimed at searching for plant biologically active individual compounds (hereinafter referred to as RBAIS) isolated from plant extracts. Aim . Validation of HPLC-UV quantitative determination of sapogenin diosgenin in plant extracts from fenugreek seeds. Materials and methods . The object of study was raw materials-fenugreek seeds produced as medicinal plant raw materials by LLC «Sage» (Irkutsk). Validation of the method was carried out according to the parameters: specificity, linearity, correctness, precision in accordance with the requirements of SP XIV. One series of medicinal plant raw materials was used for the analysis, such as serial number – 010117, release date – 15 february 2017. Results and discussion . Validation characteristics were determined and their compliance with the necessary acceptance criteria was experimentally confirmed. Conclusion . It is established that the developed method of identification and quantitative determination of diosgenin in fenugreek seed extracts by HPLC-UV is correct, precise, specific and linear in the analytical field.