Open AccessValidation of the method for determination of microbiological purity of the Caffetin® tablets
Author(s) -
Dragi Todorovic,
Ana Sokolovska,
Hristina Babunovska
Publication year - 2003
Publication title -
makedonsko farmacevtski bilten/makedonski farmacevtski bilten
Language(s) - English
Resource type - Journals
eISSN - 1857-8969
pISSN - 1409-8695
DOI - 10.33320/maced.pharm.bull.2002.48.008
Subject(s) - microorganism , candida albicans , agar , aspergillus niger , pseudomonas aeruginosa , staphylococcus aureus , food science , microbiology and biotechnology , chemistry , biology , chromatography , bacteria , genetics
A validation of the method for determination of the microbiological purity of Caffetin® tablets has been done. For this purpose the test microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus niger from the collection ATCC have been used. A non-specific nutritious medium for aerobic microorganisms and specific nutritious media for adequate test microorganisms: ENDO, Cetrimid, Baird-Parker and Sabouraud nutritious agar medium have been used. The method was performed in two modes: a direct inoculation into a nutritious medium and a membrane filtration. At the same time, a Challenge test was as well used the test of counting the growing colonies (CFU/ml). A calculation of the factor has been done, which represents relationship between growing microorganisms of the inoculated nutritious medium with and without adding to the examined preparation, as a criterion for acceptance of the achieved analytical results. The achieved values for the factor as a criterion for acceptance have shown satisfying values. It can be concluded that this method can be used for determination of the microbiological purity of Caffetin® tablets.