Open AccessDetermination of CD4+ T- Lymphocytes in Healthy Children of Kathmandu
Author(s) -
Sapana Karn,
Manjula Bhattarai,
Ramanuj Rauniyar,
Anurag Adhikari,
Pratik Karna,
Bishnu Prasad Upadhyay,
Birendra prasad Gupta
Publication year - 2018
Publication title -
journal of nepal health research council
Language(s) - English
Resource type - Journals
eISSN - 1999-6217
pISSN - 1727-5482
DOI - 10.33314/jnhrc.v16i3.1068
Subject(s) - medicine , immunophenotyping , cord blood , lymphocyte , lymphocyte subsets , reference range , population , reference values , peripheral blood , immunology , cell sorting , t cell , pediatrics , flow cytometry , immune system , environmental health
Background: The cluster differentiation (CD) of T-cell is the good marker for the immunological competence study. Nepal does not have a reference value for CD4+ T cell count and percentage for children, which severely limits the prospect of pediatric prognosis.Methods: This cross-sectional study was conducted in Kathmandu valley where total 207 children of age 0-14 year age group were recruited in this study. We analyzed 50 cord blood and 157 peripheral blood samples in order to calculate the absolute count of CD4+ T lymphocyte using Fluorescence-activated cell sorting methodology.Results: The reference range for absolute CD4+ T cell count was found to be 634-4040 cells/µL(mean1470; median: 1335 and 95% CI [1322-1617]) for male children and 491-2922 cells/µL (mean: 1443 median: 1326 and95% CI [1298-1588]) for the female children.We also observed elevated CD4 to the CD3 ratio in younger children (0.67 from cord blood Vs 0.53 from 10-14yr) compared to older ones.Conclusions: The observed CD4+ T cell counts among healthy children of Kathmandu highlights the gender differences skewed for male as well the need of defining specific reference values for other lymphocyte subsets as well in a country like Nepal which has a population with diverse genetic and socio-cultural parameters.Keywords: CD4+ T lymphocyte; children; HIV; immunophenotyping; Kathmandu; Nepal.