Open AccessIn vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid
Author(s) -
Sukhraj Kaur
Publication year - 2022
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v31i2.57343
Subject(s) - germination , activated charcoal , biology , in vitro , botany , orchidaceae , auxin , horticulture , chemistry , biochemistry , organic chemistry , adsorption , gene
The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through in vitro asymbiotic seed germination technique in Mitra orchid medium supplemented with cytokinins (Kn - 4.65 μM, BAP - 4.44 μM), and auxin (NAA- 5.37 μM). The germination frequency and initiation of germination was higher in NAA augmented medium and seedlings developed in 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication of protocorm like bodies. Activated charcoal successfully checked the release of brownish exudates in the cultures.Plant Tissue Cult. & Biotech. 31(2): 153-160, 2021 (December)