Open AccessIn vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo
Author(s) -
- Kamdem,
Néhémie T. Donfagsiteli,
Njoueretou Mfondi Mache,
Carine Temegne o,
Rodrigue Goimasse,
Leila Zebaze Zambou,
Justine Germo Nzweundji,
Emmanuel Youmbi,
Libert Brice Tonfack
Publication year - 2022
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v31i2.57342
Subject(s) - acclimatization , biology , embryo culture , germination , shoot , sodium hypochlorite , embryo , horticulture , murashige and skoog medium , botany , in vitro , tissue culture , cryopreservation , chemistry , biochemistry , organic chemistry , microbiology and biotechnology
Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida.Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)