Open AccessAgrobacterium-mediated Genetic Transformation of Rice var. BRRI Dhan 58
Author(s) -
Mst. Sufara Akhter Banu,
Boshir Ahmed,
Shahanaz Parveen,
Harun Ur Rashid,
Kazi Md. Kamrul Huda
Publication year - 2021
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v31i1.54113
Subject(s) - transformation (genetics) , callus , biology , agrobacterium tumefaciens , agrobacterium , gene , plasmid , botany , crop , gus reporter system , beta glucuronidase , horticulture , microbiology and biotechnology , gene expression , genetics , agronomy
Agrobacterium mediated genetic transformation of BRRI Dhan 58 was conducted by using immature embryos following indirect regeneration. High percentage of callus induction at 96.5% was obtained when seeds of BRRI dhan 58 were cultured on modified MS medium supplemented with 2.5 mg/l 2, 4-D under dark condition. The maximum regeneration response was rerecorded when MS was supplemented with 3 mg/l BAP + 0.5 mg/l NAA and 1.0 mg/l Kn. Genetic transformation was performed using A. tumefaciens strain LBA4404 harboring pCAMBIA1301 plasmid carrying the marker genes for β-glucuronidase (GUS) and hygromycin resistance (hptII). Integration of the GUS gene into the genome of the rice plants was confirmed by PCR. The leaf segments of the PCR positive transformed plants showed the expression of GUS. The results of this study would be an effective tool for crop improvement and functional studies of gene on rice plant.
Plant Tissue Cult. & Biotech. 31(1): 71-80, 2021 (June)