Open AccessAn Improved liquid Culture System for Efficient Shoot Multiplication in Aerva lanata (L.) Juss. Ex Schult.
Author(s) -
Kandhan Varutharaju,
Chandrasekaran Thilip,
Palusamy Raja,
Ganesan Thiagu,
Abubakker Aslam,
Apakkan Shajahan
Publication year - 2021
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v31i1.54109
Subject(s) - shoot , subculture (biology) , liquid medium , explant culture , liquid culture , micropropagation , murashige and skoog medium , botany , cytokinin , tissue culture , multiplication (music) , in vitro , horticulture , biology , chemistry , chromatography , mathematics , auxin , biochemistry , combinatorics , gene
An improved in vitro mass propagation protocol was developed for Aerva lanata using MS liquid medium. The influence of MS medium (solid and liquid) with cytokinin (TDZ and BAP, respectively) were studied for shoot proliferation and growth. The liquid medium perfomed better than solid medium in shoot multiplication. The maximum shoot multiplication rate was (29.37 ± 0.64 shoots per explant), obtained in MS liquid medium which is containing 0.6 mg/l TDZ, 0.3 mg/l NAA and 0.2 mg/l IBA. Different volumes of liquid medium have been used, 30 ml of medium flask showed the maximum number of shoots. Liquid medium is better suited for in vitro propagation of A. lanata since the enhanced multiplication rate was observed with shorter subculture intervals.
Plant Tissue Cult. & Biotech. 31(1): 35-42, 2021 (June)