Open AccessGenotypic Variability in Soybean [Glycine max (L.) Merrill] through Agrobacterium-Mediated Transformation
Author(s) -
Sangeeta Shukla,
Anita Rani,
Meeta Jain,
Vineet Kumar
Publication year - 2020
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v30i2.50693
Subject(s) - kanamycin , agrobacterium , biology , transformation (genetics) , agrobacterium tumefaciens , gus reporter system , genotype , selectable marker , epicotyl , transgene , callus , reporter gene , microbiology and biotechnology , explant culture , genetically modified crops , expression vector , gene expression , botany , gene , genetics , recombinant dna , hypocotyl , in vitro
Embryonic tip explants of 92 Indian soyabean and 7 advanced breeding lines derived from soaked mature seeds were inoculated and co-cultivated for 5-day with Agrobacterium strain EHA105 carrying the binary vector pCambia1305.1 containing a hygromycin and kanamycin resistance gene as plant and bacterial selectable markers, respectively. Transient expression of transgene was monitored by histochemical localization of β-glucouronidase (GUSPlus) reporter activity in transformed ET tissues. A high genetic variability for Agrobacterium-infection ranging from 3.8 to 100% was observed in the form of transient GUS expression. Five highly efficient genotypes, namely DS-228, JS 335, JS 72-44, KHSb2, and JS 72-280 with transient GUS expression of 100, 98.1, 96.5, 96 and 92%, respectively were identified. In addition, various infectivity patterns in these genotypes were observed. Genotypes with very high transient GUS expression identified in this study may improve success rate of development of transgenic soybean.
Plant Tissue Cult. & Biotech. 30(2): 231-242, 2020 (December)